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Current Protocols in Nucleic Acid Chemistry is a peer-reviewed journal published by Wiley, covering DNA and Nucleic Acid Chemistry, Advanced biosensing and bioanalysis techniques, RNA and protein synthesis mechanisms.
| Publisher | Wiley |
|---|---|
| Reference style | Author–year (Chicago) Author–year — (Smith, 2023) in the text Smith, Ada, Ben Jones, and Cara Lee. 2023. "A Representative Article Title." Current Protocols in Nucleic Acid Chemistry 12 (3): 45–58.
Formats any DOI in Current Protocols in Nucleic Acid Chemistry style. No sign-up. |
| Publishes research in | DNA and Nucleic Acid Chemistry Advanced biosensing and bioanalysis techniques RNA and protein synthesis mechanisms RNA Interference and Gene Delivery HIV/AIDS drug development and treatment |
| ISSN | 1934-9270 |
| h-index | 34 |
| i10-index | 122 |
| Total citations | 4,798 |
| Article processing charge | $5,050 |
| Top institutions publishing here | Vanderbilt University |
| Journal website | onlinelibrary.wiley.com |
| You get | A submission-ready PDF and the editable LaTeX source — ready to submit. |
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Abstract Absorbance versus temperature curves can provide information on the thermal stability of DNA or RNA quadruplexes. Quadruplex denaturation or renaturation can be followed by recording absorbance at 295 nm rather than 260 nm, the wavelength used to monitor duplex denaturation. This unit describes the use of absorbance versus temperature curves to determine melting temperatures…
Details for predicting secondary structure of RNA sequences using free energy minimization are given. Protocols present the use of the RNAstructure computer program (for PCs) and the mfold server (for Unix platforms). The minimum free energy structure and a set of suboptimal structures with similar free energies are predicted. Prediction of high-affinity oligonucleotide binding sites…
Although DNA is most widely known for its ability to store and pass along genetic information, the discovery of G-quadruplex structures has illuminated a new role for DNA in biology. DNA G-quadruplexes are four-stranded globular nucleic acid secondary structures formed in specific G-rich sequences with biological significance, such as human telomeres and oncogene promoters. This…
This unit provides thorough coverage of the most useful chemical and enzyme probes that can be used to examine RNA secondary and tertiary structure. Footprinting methods are presented using dimethyl sulfate, diethyl pyrocarbonate, ethylnitrosourea, kethoxal, CMCT, and nucleases. For chemical probes, both strand scission and primer extension detection protocols are included.
Circular dichroism (CD) is a phenomenon that arises from the differential absorption of left- and right-handed circularly polarized light, and may be seen with optically active molecules. CD spectroscopy provides useful spectral signatures for biological macromolecules in solution, and provides low-resolution structural information about macromolecular conformation. CD spectroscopy is particularly useful for monitoring conformational changes…
