An important recent advance in the functional analysis of Saccharomyces cerevisiae genes is the development of the one-step PCR-mediated technique for deletion and modification of chromosomal genes. This method allows very rapid gene manipulations without requiring plasmid clones of the gene of interest. We describe here a new set of plasmids that serve as templates…
Yeast Template
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About the Yeast format
Yeast is a peer-reviewed journal published by Wiley, covering Fungal and yeast genetics research, RNA and protein synthesis mechanisms, Fermentation and Sensory Analysis.
| Publisher | Wiley |
|---|---|
| Reference style | Author–year (Chicago) Author–year — (Smith, 2023) in the text Smith, Ada, Ben Jones, and Cara Lee. 2023. "A Representative Article Title." Yeast 12 (3): 45–58.
Formats any DOI in Yeast style. No sign-up. |
| Publishes research in | Fungal and yeast genetics research RNA and protein synthesis mechanisms Fermentation and Sensory Analysis Yeasts and Rust Fungi Studies Microbial Metabolic Engineering and Bioproduction |
| ISSN | 0749-503X |
| Citation impact (2-yr) | 1.38 |
| h-index | 153 |
| i10-index | 2,482 |
| Total citations | 164,200 |
| Article processing charge | $3,660 |
| Top institutions publishing here | Centre National de la Recherche Scientifique |
| Journal website | onlinelibrary.wiley.com |
| You get | A submission-ready PDF and the editable LaTeX source — ready to submit. |
Papers published in Yeast per year
Citation impact of Yeast by publication year
Citations each year’s papers have accumulated so far — the most recent years are still building up.
Most-cited papers in Yeast
A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains minimize or eliminate the homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. Because…
We have constructed and tested a dominant resistance module, for selection of S. cerevisiae transformants, which entirely consists of heterologous DNA. This kanMX module contains the known kanr open reading-frame of the E. coli transposon Tn903 fused to transcriptional and translational control sequences of the TEF gene of the filamentous fungus Ashbya gossypii. This hybrid…
We describe a straightforward PCR-based approach to the deletion, tagging, and overexpression of genes in their normal chromosomal locations in the fission yeast Schizosaccharomyces pombe. Using this approach and the S. pombe ura4+ gene as a marker, nine genes were deleted with efficiencies of homologous integration ranging from 6 to 63%. We also constructed a…
Tagging of genes by chromosomal integration of PCR amplified cassettes is a widely used and fast method to label proteins in vivo in the yeast Saccharomyces cerevisiae. This strategy directs the amplified tags to the desired chromosomal loci due to flanking homologous sequences provided by the PCR-primers, thus enabling the selective introduction of any sequence…