The protocol described in this appendix allows for light microscopic quantitation of cell viability. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cells).
Current Protocols in Immunology Template
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About the Current Protocols in Immunology format
Current Protocols in Immunology is a peer-reviewed journal published by Wiley, covering Immune Cell Function and Interaction, T-cell and B-cell Immunology, Monoclonal and Polyclonal Antibodies Research.
| Publisher | Wiley |
|---|---|
| Reference style | Author–year (Chicago) Author–year — (Smith, 2023) in the text Smith, Ada, Ben Jones, and Cara Lee. 2023. "A Representative Article Title." Current Protocols in Immunology 12 (3): 45–58.
Formats any DOI in Current Protocols in Immunology style. No sign-up. |
| Publishes research in | Immune Cell Function and Interaction T-cell and B-cell Immunology Monoclonal and Polyclonal Antibodies Research Immunotherapy and Immune Responses Immune Response and Inflammation |
| ISSN | 1934-3671 |
| h-index | 83 |
| i10-index | 425 |
| Total citations | 37,053 |
| Article processing charge | $5,050 |
| Top institutions publishing here | National Institute of Allergy and Infectious Diseases |
| Journal website | currentprotocols.onlinelibrary.wiley.com |
| You get | A submission-ready PDF and the editable LaTeX source — ready to submit. |
Papers published in Current Protocols in Immunology per year
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Most-cited papers in Current Protocols in Immunology
Inflammatory bowel diseases (IBD), mainly comprising ulcerative colitis and Crohn's Disease, are complex and multifactorial diseases with unknown etiology. For the past 20 years, to study human IBD mechanistically, a number of murine models of colitis have been developed. These models are indispensable tools to decipher underlying mechanisms of IBD pathogenesis as well as to…
Macrophages are mononuclear phagocytes that are widely distributed throughout the body. These cells can contribute to development and homeostasis and participate in innate and adaptive immune responses. The physiology of macrophages can vary tremendously depending on the environment in which they reside and the local stimuli to which they are exposed. Macrophages are prodigious secretory…
The dye exclusion test is used to determine the number of viable cells present in a cell suspension. It is based on the principle that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not. In this test, a cell suspension is simply…
The 4T1 mammary carcinoma is a transplantable tumor cell line that is highly tumorigenic and invasive and, unlike most tumor models, can spontaneously metastasize from the primary tumor in the mammary gland to multiple distant sites including lymph nodes, blood, liver, lung, brain, and bone The 4T1 tumor has several characteristics that make it a…