The copper-catalyzed azide-alkyne cycloaddition reaction is widely used for the connection of molecular entities of all sizes. A protocol is provided here for the process with biomolecules. Ascorbate is used as reducing agent to maintain the required cuprous oxidation state. Since these convenient conditions produce reactive oxygen species, five equivalents of a copper-binding ligand is…
Current Protocols in Chemical Biology Template
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About the Current Protocols in Chemical Biology format
Current Protocols in Chemical Biology is a peer-reviewed journal published by Wiley, covering Click Chemistry and Applications, Advanced biosensing and bioanalysis techniques, RNA and protein synthesis mechanisms.
| Publisher | Wiley |
|---|---|
| Reference style | Author–year (Chicago) Author–year — (Smith, 2023) in the text Smith, Ada, Ben Jones, and Cara Lee. 2023. "A Representative Article Title." Current Protocols in Chemical Biology 12 (3): 45–58.
Formats any DOI in Current Protocols in Chemical Biology style. No sign-up. |
| Publishes research in | Click Chemistry and Applications Advanced biosensing and bioanalysis techniques RNA and protein synthesis mechanisms Chemical Synthesis and Analysis Monoclonal and Polyclonal Antibodies Research |
| ISSN | 2160-4762 |
| h-index | 31 |
| i10-index | 102 |
| Total citations | 4,290 |
| Article processing charge | $5,050 |
| Top institutions publishing here | Harvard University |
| Journal website | currentprotocols.onlinelibrary.wiley.com |
| You get | A submission-ready PDF and the editable LaTeX source — ready to submit. |
Papers published in Current Protocols in Chemical Biology per year
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Most-cited papers in Current Protocols in Chemical Biology
Drug Affinity Responsive Target Stability is a general methodology for identifying and studying protein-ligand interactions. The technique is based on the principle that when a small molecule compound binds to a protein, the interaction stabilizes the target protein's structure such that it becomes protease resistant. DARTS is particularly useful for the initial identification of the…
Cyclic Immunofluorescence (CycIF) is a public-domain method for performing highly multiplexed immunofluorescence imaging using a conventional epifluorescence microscope. It uses simple reagents and existing antibodies to construct images with up to 30 channels by sequential 4- to 6-channel imaging followed by fluorophore inactivation. Three variant methods are described, the most generally useful of which involves…
The technique of fluorescence polarization (FP) is based on the observation that when a fluorescently labeled molecule is excited by polarized light, it emits light with a degree of polarization that is inversely proportional to the rate of molecular rotation. This property of fluorescence can be used to measure the interaction of a small labeled…
Target identification of biologically active small-molecules is often the rate-determining step in forward chemical genetics. Photo-affinity labeling (PAL) represents a useful biochemical strategy for target identification in complex protein mixtures. This unit describes the use of alkyl diazirine-based photo-affinity probes and Cu(I)-catalyzed click chemistry to covalently label and visualize the targets of biologically active small-molecules.…