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Methods and Protocols is a peer-reviewed journal published by MDPI, covering CRISPR and Genetic Engineering, Molecular Biology Techniques and Applications, RNA and protein synthesis mechanisms. Over its lifetime it has published 903 papers, which have received 8,173 citations.
| Publisher | MDPI |
|---|---|
| Reference style | Numbered (MDPI) Numbered — [1], [2] in the text 1. Smith, A.; Jones, B.; Lee, C. A Representative Article Title. Methods and Protocols 2023, 12, 45–58.
Formats any DOI in Methods and Protocols style. No sign-up. |
| Abbreviation (ISO-4) | Methods Protoc. |
| Publishes research in | CRISPR and Genetic Engineering Molecular Biology Techniques and Applications RNA and protein synthesis mechanisms 3D Printing in Biomedical Research Advanced biosensing and bioanalysis techniques |
| ISSN | 2409-9279 |
| Citation impact (2-yr) | 1.99 |
| h-index | 36 |
| i10-index | 227 |
| Article processing charge | $1,732 |
| Open access | Yes — indexed in DOAJ |
| Top institutions publishing here | Sapienza University of Rome |
| Journal website | www.mdpi.com |
| You get | A submission-ready PDF and the editable LaTeX source — ready to submit. |
Citations each year’s papers have accumulated so far — the most recent years are still building up.
MicroRNAs (miRNAs) represent a family of short non-coding regulatory RNA molecules that are produced in a tissue and time-specific manner to orchestrate gene expression post-transcription. MiRNAs hybridize to target mRNA(s) to induce translation repression or mRNA degradation. Functional studies have demonstrated that miRNAs are engaged in virtually every physiological process and, consequently, miRNA dysregulations have…
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more. The advantages of CFPS over in vivo protein expression include its open system, the elimination of reliance on living cells, and the ability to focus all system energy on production of the protein…
MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression in both animals and plants. By pairing to microRNA responsive elements (mREs) on target mRNAs, miRNAs play gene-regulatory roles, producing remarkable changes in several physiological and pathological processes. Thus, the identification of miRNA-mRNA target interactions is fundamental for discovering the regulatory network governed by miRNAs. The best…
Pseudotype neutralization assays are powerful tools to study functional antibody responses against viruses in low biosafety laboratories. However, protocols described in the literature differ widely with respect to material, reagents, and methods used to perform these assays and to analyse the raw data generated. This could result in discrepancies between the results of different laboratories…
Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria. As part of gaining a better understanding of phages and to determine if phages harbor putative virulence factors, whole genome sequencing is used, for which good quality phage DNA is needed.…
