BACKGROUND: We describe novel plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves. We have generated a series of pGreenII cloning vectors that are ideally suited to transient gene expression, by removing elements of conventional binary vectors necessary for stable transformation such as transformation selection genes. RESULTS: We give an example…
Plant Methods Template
Write in a clean editor, then format for Plant Methods in one click — DocuGuru applies the official BMC (BioMed Central) template with numbered references and exports a submission-ready PDF plus the editable LaTeX source. Free to start.
About the Plant Methods format
Plant Methods is a peer-reviewed journal published by BMC (BioMed Central), covering Plant Molecular Biology Research, Smart Agriculture and AI, Remote Sensing in Agriculture. Over its lifetime it has published 1,841 papers, which have received 82,579 citations.
| Publisher | BMC (BioMed Central) |
|---|---|
| Reference style | Numbered (Vancouver) Numbered — [1], [2] in the text 1. Smith A, Jones B, Lee C. A representative article title. Plant Methods. 2023;12(3):45–58.
Formats any DOI in Plant Methods style. No sign-up. |
| Publishes research in | Plant Molecular Biology Research Smart Agriculture and AI Remote Sensing in Agriculture Plant tissue culture and regeneration Plant nutrient uptake and metabolism |
| ISSN | 1746-4811 |
| Citation impact (2-yr) | 6.3 |
| h-index | 134 |
| i10-index | 1,241 |
| Article processing charge | $2,195 |
| Open access | Yes — indexed in DOAJ |
| Top institutions publishing here | Nanjing Agricultural University |
| Journal website | plantmethods.biomedcentral.com |
| You get | A submission-ready PDF and the editable LaTeX source — ready to submit. |
Papers published in Plant Methods per year
Citation impact of Plant Methods by publication year
Citations each year’s papers have accumulated so far — the most recent years are still building up.
Most-cited papers in Plant Methods
Over the last 10 years, high-density SNP arrays and DNA re-sequencing have illuminated the majority of the genotypic space for a number of organisms, including humans, maize, rice and Arabidopsis. For any researcher willing to define and score a phenotype across many individuals, Genome Wide Association Studies (GWAS) present a powerful tool to reconnect this…
Plastome (plastid genome) sequences provide valuable information for understanding the phylogenetic relationships and evolutionary history of plants. Although the rapid development of high-throughput sequencing technology has led to an explosion of plastome sequences, annotation remains a significant bottleneck for plastomes. User-friendly batch annotation of multiple plastomes is an urgent need. We introduce Plastid Genome Annotator…
MicroRNAs (miRNAs) are a class of small non-coding RNAs with a critical role in development and environmental responses. Efficient and reliable detection of miRNAs is an essential step towards understanding their roles in specific cells and tissues. However, gel-based assays currently used to detect miRNAs are very limited in terms of throughput, sensitivity and specificity.…
BACKGROUND: Plant protoplasts, a proven physiological and versatile cell system, are widely used in high-throughput analysis and functional characterization of genes. Green protoplasts have been successfully used in investigations of plant signal transduction pathways related to hormones, metabolites and environmental challenges. In rice, protoplasts are commonly prepared from suspension cultured cells or etiolated seedlings, but…