Abstract We describe two methods for detecting acrosome reactions of human sperm at the light microscopic level. The techniques include the use of a supravital stain to detect dead sperm in order to differentiate between “physiological” and “degenerative” acrosome reactions. Sperm are incubated with the supravital stain Hoechst 33258 (a fluorescent DNA‐binding dye with limited…
Gamete Research Template
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About the Gamete Research format
Gamete Research is a peer-reviewed journal published by Wiley, covering Sperm and Testicular Function, Reproductive Biology and Fertility, Ovarian function and disorders.
| Publisher | Wiley |
|---|---|
| Reference style | Author–year (Chicago) Author–year — (Smith, 2023) in the text Smith, Ada, Ben Jones, and Cara Lee. 2023. "A Representative Article Title." Gamete Research 12 (3): 45–58.
Formats any DOI in Gamete Research style. No sign-up. |
| Publishes research in | Sperm and Testicular Function Reproductive Biology and Fertility Ovarian function and disorders Reproductive biology and impacts on aquatic species Marriage and Sexual Relationships |
| ISSN | 0148-7280 |
| h-index | 83 |
| i10-index | 619 |
| Total citations | 30,486 |
| Top institutions publishing here | University of California, Davis |
| Journal website | onlinelibrary.wiley.com |
| You get | A submission-ready PDF and the editable LaTeX source — ready to submit. |
Papers published in Gamete Research per year
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Most-cited papers in Gamete Research
Mouse and human spermatozoa, but not rabbit spermatozoa, have long been known to be sensitive to loss of motility induced by exogenous H2O2. Recent work has shown that loss of sperm motility in these species correlates with the extent of spontaneous lipid peroxidation. In this study, the effect of H2O2 on this reaction in sperm…
Abstract The abundance of data pertaining to the metabolism of lipids in relation to mammalian fertilization has warranted an effort to assemble a molecular membrane model for the comprehensive visualization of the biochemical events involved in sperm capacitation and the acrosome reaction. Derived both from earlier models as well as from current concepts, our membrane…
Bovine cumulus oocyte complexes (COCs) as used for in vitro maturation and fertilization can be classified into different categories by light microscopical inspection. We have distinguished four categories based on compactness and transparency of the cumulus investment and homogeneity and transparency of the ooplasm. The four categories were studied for their morphological characteristics at the…
The formation of malondialdehyde (MDA), a product of lipid peroxidation (LPO), was measured in human spermatozoa from 27 subjects with normal sperm characteristics. Peroxidation of lipids in washed spermatozoa was induced by catalytic amounts of ferrous ions and ascorbate and malondialdehyde determined by thiobarbituric method. MDA formation varied considerably from one sample to another. The…